| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169068 |
| Chromosome: | chromosome_4 |
| Location: | 335521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217908 | FAP79 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAGCTGTGGGGTGTGCGTGCGTGTGCATG |
| Internal bar code: | ACGCGCCGCGTGATGACCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 504 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATACCGCACGAAAGAACC |
| Suggested primer 2: | CACTCGGCAAGCTATGTTCA |