Insertion junction: LMJ.RY0402.169116_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.169116
Chromosome:chromosome 5
Location:2905015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g238100 PTK25 (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGTGGTGAGCCATCCTCGATGACGCCC
Internal bar code:TCTGTGTCCTGACTCATATGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:897
LEAP-Seq percent confirming:99.5415
LEAP-Seq n confirming:1737
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACATCCTGGGTCTGGTT
Suggested primer 2:CCACCACGAGCTAAAGAAGC