| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169116 |
| Chromosome: | chromosome 5 |
| Location: | 2905044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238100 | PTK25 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGATTCGAAGGGGGTCCGCGTATGACG |
| Internal bar code: | TTACGCAGGTACTGTTGCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 94.7729 |
| LEAP-Seq n confirming: | 1523 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACATCCTGGGTCTGGTT |
| Suggested primer 2: | CCACCACGAGCTAAAGAAGC |