| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169118 |
| Chromosome: | chromosome 12 |
| Location: | 3320928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497750 | TSPP1,TPS2 | Trehalose 6-phosphate phosphatase; (1 of 1) K01087 - trehalose 6-phosphate phosphatase (otsB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCTTGACACACGACATACATCCAGATT |
| Internal bar code: | TGGCCCTTTCTGGGGATGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.6455 |
| LEAP-Seq n confirming: | 4216 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTAGCGAACCAAAAAGG |
| Suggested primer 2: | ATCGTGGAGTGGTCCAAAAG |