| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169157 |
| Chromosome: | chromosome 12 |
| Location: | 9237165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTGTTTCACCGCACCGTTTCGTCAGC |
| Internal bar code: | AACTCCAGTCTTTCCCGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 99.5335 |
| LEAP-Seq n confirming: | 6187 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGTAAAGGCTGTGGAAA |
| Suggested primer 2: | TGGGGAGAAGCCATCAATAG |