| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169203 |
| Chromosome: | chromosome 6 |
| Location: | 8041696 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304250 | RLS2 | RegA/Rls-like protein 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGAGCTGCTGCCGCCGCCGCCGCCCCT |
| Internal bar code: | CCTCACAGGCTCTAGTCGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 300 |
| LEAP-Seq n nonconfirming: | 250 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCGTAGGCGATAGCTG |
| Suggested primer 2: | TAGACTGCCCGTTAACGCTT |