| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169214 |
| Chromosome: | chromosome 10 |
| Location: | 1932461 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432050 | FAP284 | Flagellar Associated Protein 284; (1 of 2) PF15239 - Domain of unknown function (DUF4586) (DUF4586) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTCATTACAGTGGGAATGCACGGTGGA |
| Internal bar code: | CTGGGATCTGTGAAGTTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 89.6454 |
| LEAP-Seq n confirming: | 632 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACGTAGGCAGCACCTC |
| Suggested primer 2: | GGCAAGCCAACATACAACCT |