| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169227 |
| Chromosome: | chromosome 7 |
| Location: | 4104302 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCGCCAAATGATCACACGCGCGTGTGA |
| Internal bar code: | GTCAGCGAGGGACCCGGAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 70.6768 |
| LEAP-Seq n confirming: | 8072 |
| LEAP-Seq n nonconfirming: | 3349 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTGATCTCACAGTCCA |
| Suggested primer 2: | ACACACACACACACACACCG |