Insertion junction: LMJ.RY0402.169237_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.169237
Chromosome:chromosome 1
Location:4476114
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g030450 (1 of 1) IPR006615//IPR028881//IPR028889//IPR029071 - Peptidase C19, ubiquitin-specific peptidase, DUSP domain // PAN2 domain // Ubiquitin specific protease domain // Ubiquitin-related domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACAAACGACGTGGCAGCTCTTGCGCAG
Internal bar code:AAGTTGGGAGGCGCGTTCAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:815
LEAP-Seq percent confirming:99.8249
LEAP-Seq n confirming:2850
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGCTTCAAGAACACTGC
Suggested primer 2:CGTCGCTTAGGAAGAACCTG