| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169243 |
| Chromosome: | chromosome 6 |
| Location: | 5576260 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285500 | (1 of 2) K13173 - arginine and glutamate-rich protein 1 (ARGLU1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGAGCGACGGTGTTTGGGGGTCGCCGG |
| Internal bar code: | GGTCCATCCGTTGTGGACTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 2.79279 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1079 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACGGCACATACATACGC |
| Suggested primer 2: | GTTCGAGGAGCTACGTCAGG |