| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169269 |
| Chromosome: | chromosome 8 |
| Location: | 1534228 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364862 | (1 of 1) PF07714//PF13185 - Protein tyrosine kinase (Pkinase_Tyr) // GAF domain (GAF_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTGCTATCTGCTTCATGCTTTGGATTA |
| Internal bar code: | AATCCTGAGTTACTGACTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.7792 |
| LEAP-Seq n confirming: | 2259 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGCAGTAGAAGGCAGC |
| Suggested primer 2: | GTTGATGAGGAGGAGTTCGG |