| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169287 |
| Chromosome: | chromosome 5 |
| Location: | 1732686 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242700 | SUB9 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTATTCACGTGCTTCACAGGCGCTGGGA |
| Internal bar code: | GACGGGGTCGTCGCGGCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 97.3384 |
| LEAP-Seq n confirming: | 1024 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTAGCCAGTTCAACACC |
| Suggested primer 2: | CGCGTGTTAATCACATCACC |