| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169290 |
| Chromosome: | chromosome 9 |
| Location: | 1764285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396450 | APT3,GOX15 | Acid phosphatase; (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTGATTGAGGAAAACAGTCAGTGCGGC |
| Internal bar code: | ATCCAGTGAGTCGCCGGAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.8211 |
| LEAP-Seq n confirming: | 3906 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAGTAGAGGAAGTGCG |
| Suggested primer 2: | CGAGCAGATTCATCTGGGAT |