| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169290 |
| Chromosome: | chromosome 9 |
| Location: | 1764298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396450 | APT3,GOX15 | Acid phosphatase; (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTGCAGCCCTTCCACTGCCCGTTGCC |
| Internal bar code: | TAGGCCTTGATTTTGTGACCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.6419 |
| LEAP-Seq n confirming: | 14746 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAGTAGAGGAAGTGCG |
| Suggested primer 2: | CGAGCAGATTCATCTGGGAT |