| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169298 |
| Chromosome: | chromosome 3 |
| Location: | 517969 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145267 | (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACATGCAACATTTCCCCGAAGTGGACT |
| Internal bar code: | GCCAAAGGGTTTTGGGAGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.559 |
| LEAP-Seq n confirming: | 9255 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTACTGTACGCTTTCCCA |
| Suggested primer 2: | TCGTGAGAGCGTGGTGATAG |