| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169308 |
| Chromosome: | chromosome 12 |
| Location: | 3126612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499750 | (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGGAGTTGGGCTTATAGCACTCAGGT |
| Internal bar code: | AGAGCATTCTCCTCGCCCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 98.513 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATGTTGGTCAGGGCTC |
| Suggested primer 2: | GTCAACCGGTCAAATGTGTG |