| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169312 |
| Chromosome: | chromosome 10 |
| Location: | 3352078 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443900 | BOR1 | (1 of 1) PF00955 - HCO3- transporter family (HCO3_cotransp); Borate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCTACACCCCCTGCATTCTGTACCGCT |
| Internal bar code: | CAGTTAGTCGTTGATTACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 99.6234 |
| LEAP-Seq n confirming: | 529 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAGCATAAGACGCTGG |
| Suggested primer 2: | GTGGAAGGAGCAGTCCAGAG |