| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169320 |
| Chromosome: | chromosome 17 |
| Location: | 2689877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717850 | PHC8 | (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCCACTGCGCCGCGGGGCCTTTCTTT |
| Internal bar code: | GTCGTAGGTGGAGACGTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 98.5965 |
| LEAP-Seq n confirming: | 4777 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGACAGCACCGTCTGTTG |
| Suggested primer 2: | CTGACGACCCCGTATTGTCT |