| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169322 |
| Chromosome: | chromosome 6 |
| Location: | 5534298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGACTCGTCCTGTCCCGCCCTGCTACT |
| Internal bar code: | AAGTTACCCATTGTGCGGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 83.3068 |
| LEAP-Seq n confirming: | 524 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACCCACTGTAGCACACA |
| Suggested primer 2: | ACGATTAATGTTCCCCACGA |