| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169337 |
| Chromosome: | chromosome_16 |
| Location: | 6801226 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g674515 | IDA7 | Flagellar inner dynein arm I1 intermediate chain IC140 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGGGATTGGTGGCAGGTCACGGACCGG |
| Internal bar code: | GGGGGCGGCGGCGTAATGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 517 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTAACCCTGCTACTGCG |
| Suggested primer 2: | GGACACCCAACTCGCAGTAT |