| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169377 |
| Chromosome: | chromosome 13 |
| Location: | 3439744 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587450 | FCL2 | Predicted 5-formyltetrahydrofolate cycloligase; (1 of 1) PTHR13017:SF0 - METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTGGAGCACGACGTGCCTGTGGACAT |
| Internal bar code: | CGACTTTTGCGCAGACTATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 99.7297 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCATGACCCTTCACGACT |
| Suggested primer 2: | AGGTGGATCTCATCGTGGTC |