| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169385 |
| Chromosome: | chromosome 4 |
| Location: | 1436352 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214250 | AGO2 | Argonaute-like protein; (1 of 3) PTHR22891:SF0 - PROTEIN ARGONAUTE-2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCCAAATCTCTTCCGCTGTGTGCCTCA |
| Internal bar code: | TTGAGCTCACACCAAACACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.5618 |
| LEAP-Seq n confirming: | 7725 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGTGCGTAAATCTGA |
| Suggested primer 2: | GAGAGCTTGGTTGTTCGGAG |