Insertion junction: LMJ.RY0402.169396_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.169396
Chromosome:chromosome 6
Location:7945429
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g303483 (1 of 5) IPR001005//IPR009057//IPR017877 - SANT/Myb domain // Homeodomain-like // Myb-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGACTCAACTAGCGCCCATGCCACCAG
Internal bar code:AAGGGAACGACCGACGTGGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1020
LEAP-Seq percent confirming:99.9442
LEAP-Seq n confirming:1790
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATCAGCGTAAATCAGCG
Suggested primer 2:GCTAACAACTGCCAATGCAA