| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169411 |
| Chromosome: | chromosome 10 |
| Location: | 4279339 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450600 | (1 of 1) PTHR10024 - SYNAPTOTAGMIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATTTGATCATTGCTTAGTGCGTGCGCG |
| Internal bar code: | GAGCTTCAGCGTTGCGGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 476 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTAAGCAGACAGCAAACA |
| Suggested primer 2: | TCTGTCACATGCCTTTGAGG |