| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169416 |
| Chromosome: | chromosome_2 |
| Location: | 3650359 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g095149 | FAP143 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCTGAATTTAAGCGTGCAACATAATATGT |
| Internal bar code: | TGTTAGTCTAAGGGAGTAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 99.2308 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACATTTGTTGCCTGGTG |
| Suggested primer 2: | CCCAGTCTGAGCTGAGTTCC |