| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169448 |
| Chromosome: | chromosome 1 |
| Location: | 5085275 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035300 | CPA2 | (1 of 2) 3.5.1.53 - N-carbamoylputrescine amidase; N-carbamoylputrescine amidase | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGATACCTTGAGCCGTCTCCGCCAGC |
| Internal bar code: | GGATGCGGCCCAGTGTACATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 99.7842 |
| LEAP-Seq n confirming: | 3699 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAAGACTTCCCCATTTGC |
| Suggested primer 2: | CAAGGTGGTGTTTGACATGC |