| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169458 |
| Chromosome: | chromosome 3 |
| Location: | 5198189 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182551 | PCY1,PC6-2 | Pre-apoplastocyanin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAGACCGTGAACTTCGTGAACAACGCT |
| Internal bar code: | CGCGAGTACGACATGTCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.2388 |
| LEAP-Seq n confirming: | 1434 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACCGAGTAGGTCTCGC |
| Suggested primer 2: | CGTGCAGCTACCTTTCATCA |