| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169469 |
| Chromosome: | chromosome 2 |
| Location: | 526782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077100 | GSH1 | (1 of 1) 6.3.2.2 - Glutamate--cysteine ligase / Gamma-glutamylcysteine synthetase; Gamma-glutamylcysteine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAACGGTGACAGGGACGGGTCTGGGGC |
| Internal bar code: | GCGTAGCGATAGTCGAGCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 99.4394 |
| LEAP-Seq n confirming: | 4967 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCACAGAGATGGACAGA |
| Suggested primer 2: | ATCGGTATGTCGGAAAGACG |