| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169499 |
| Chromosome: | chromosome 17 |
| Location: | 3302147 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723200 | FAP372 | Flagellar Associated EF Hand Protein 372 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGGGTGCAAACTGGCTTGCATAGGTA |
| Internal bar code: | GCGGACAGCGCTGAAGATCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 94.0823 |
| LEAP-Seq n confirming: | 3291 |
| LEAP-Seq n nonconfirming: | 207 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAGTCGAAGCTCAAAATC |
| Suggested primer 2: | GCCGGTTATTGCAAGTTTGT |