| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169515 |
| Chromosome: | chromosome 14 |
| Location: | 2050458 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAAAGACGCGGCCGCAGTAGTACTCAAT |
| Internal bar code: | GGGGACGTACCGGCCTGGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 24.8243 |
| LEAP-Seq n confirming: | 565 |
| LEAP-Seq n nonconfirming: | 1711 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTAGCATCACAGACACA |
| Suggested primer 2: | CACTCACAGGTGCAGGAGAG |