Insertion junction: LMJ.RY0402.169528_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.169528
Chromosome:chromosome 9
Location:4172900
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394028 (1 of 21) IPR000679 - Zinc finger, GATA-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTCCGCGCGGGGCTGTGGGGCTTCGCG
Internal bar code:TCCGCATAGTACTTCGGGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:99.4737
LEAP-Seq n confirming:2079
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGATGTGGATATGTCGGA
Suggested primer 2:CTCTCTCCGTTGTCGCTACC