| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169542 |
| Chromosome: | chromosome 4 |
| Location: | 1597044 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAAGAAGGGGGGGTGAGAGTCAGGGGAA |
| Internal bar code: | GAGTGGCATAGCGGGCGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGAGGAGGGTTCAATTA |
| Suggested primer 2: | ACGATTATGAAGGACGGTGC |