| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169616 |
| Chromosome: | chromosome 11 |
| Location: | 2715550 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTGCGTACTCATTGCCAACACACGCCA |
| Internal bar code: | AGGCGGCCGGACGGTGTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 82.9529 |
| LEAP-Seq n confirming: | 2253 |
| LEAP-Seq n nonconfirming: | 463 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTTGCACATCGACAGA |
| Suggested primer 2: | TAAAAGGACGCACCGTTACC |