| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169631 |
| Chromosome: | chromosome 5 |
| Location: | 1597103 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243453 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCGGAGAGCTAGCGATGACCGCAAAAC |
| Internal bar code: | AGTCGTTATGGCATTCTCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 99.8755 |
| LEAP-Seq n confirming: | 802 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCGAGCGGCTCATATTT |
| Suggested primer 2: | ACTGGATGGCTTCGAGCTAA |