| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169646 |
| Chromosome: | chromosome 10 |
| Location: | 2484696 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436150 | COA6,COAE1 | (1 of 1) K00859 - dephospho-CoA kinase (coaE); Dephospho-CoA kinase, CoA biosynthesis | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGGGACCCTCACCGCTGCACCTGCCAT |
| Internal bar code: | CGTGCTTTTCCCTACCGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.8564 |
| LEAP-Seq n confirming: | 2086 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATCTGGACACACCGTGC |
| Suggested primer 2: | ACTGGAGGGACTGTGTGGAC |