Insertion junction: LMJ.RY0402.169653_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.169653
Chromosome:chromosome 12
Location:2562825
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g505400 (1 of 1) 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase / Platelet-activating factor acetylhydrolase; Related to platelet-activating factor acetylhydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGTGGCGTCAACAGCGCGCCAGTATGTG
Internal bar code:TACGGTGTAACCGTGCTAGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:385
LEAP-Seq percent confirming:99.5969
LEAP-Seq n confirming:4447
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGTGTGCTTCCCTGTAT
Suggested primer 2:CCCTTAGTGGCGTAGAGCTG