| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169703 |
| Chromosome: | chromosome 6 |
| Location: | 3024466 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273900 | HTA41 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCACCTTCAGGGGTGCCTACCTGCTCT |
| Internal bar code: | CCGTCAGAGGGCGTTCGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 91.0037 |
| LEAP-Seq n confirming: | 2974 |
| LEAP-Seq n nonconfirming: | 294 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCACGCTACCTGAAGA |
| Suggested primer 2: | ACCCTGACACGTATTGCCTC |