| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169721 |
| Chromosome: | chromosome 2 |
| Location: | 2370583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091000 | (1 of 1) PF05346 - Eukaryotic membrane protein family (DUF747) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTGAGCGCCTCTAGCACGTCGTTGCC |
| Internal bar code: | GGTTAGTACCCCCTCGAACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 1911 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGCTCAAGGACATCACC |
| Suggested primer 2: | GGAGGTGTGAGAGGGCATAA |