| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169732 |
| Chromosome: | chromosome 3 |
| Location: | 4046289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172350 | (1 of 1) PF14736 - Protein N-terminal asparagine amidohydrolase (N_Asn_amidohyd) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCTTGAGCCAGTGGCACGTGGGTAGCG |
| Internal bar code: | GTTCGTCCGGAATACCGACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 99.1824 |
| LEAP-Seq n confirming: | 3154 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGGTTTGGTGGTGGAT |
| Suggested primer 2: | AGCATGACTTTGTGCCCTCT |