| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169756 |
| Chromosome: | chromosome 11 |
| Location: | 241100 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467555 | (1 of 1) PF00023//PF13857 - Ankyrin repeat (Ank) // Ankyrin repeats (many copies) (Ank_5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCTACATGGGCGTGGACTTGTAGCTA |
| Internal bar code: | GTTAACAAGGTGAAATCATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 64.4444 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTATGGATAGGAGGCGT |
| Suggested primer 2: | TTCTGTCACGCCTGTCTGTC |