| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169766 |
| Chromosome: | chromosome 12 |
| Location: | 4442093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521300 | POB14 | Proteome of basal body 14 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAAGCGAGGCTATGCGAACGTGTAGCA |
| Internal bar code: | CGGTGGTCGGGTTCTCCAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.3879 |
| LEAP-Seq n confirming: | 1299 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACATAACAAGCATCACG |
| Suggested primer 2: | CATGCGCAACTAAGCTACCA |