| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169770 |
| Chromosome: | chromosome_9 |
| Location: | 5895393 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g402515 | AMX1 | Copper amine oxidase | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCAATTCCGCGACAGCCCTTCCAGCGAG |
| Internal bar code: | TCCTTGGCTGCAGCTGGCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 99.3794 |
| LEAP-Seq n confirming: | 2562 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATCGCGAGAAAGAAGTC |
| Suggested primer 2: | CTCGGTCGGCTTTTTCATAG |