Insertion junction: LMJ.RY0402.169783_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.169783
Chromosome:chromosome 17
Location:4128752
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCGGCGAGGGGCTGACCCACGAGCAGG
Internal bar code:GGACTATGGCGTCGTGGCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:26
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:273
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCAATCGATGCTTTGTAGCG
Suggested primer 2:CAATACCGTCAGCACCACAC