| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169794 |
| Chromosome: | chromosome 5 |
| Location: | 1251451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245352 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGTGGCCCAACTGGCAGTCGACCACGC |
| Internal bar code: | TTCAGTCATGAATTCTCTAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 99.5154 |
| LEAP-Seq n confirming: | 1848 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCCTTCGCCACCACTAC |
| Suggested primer 2: | GTGATGGGTTGCGAAAAGTT |