| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169803 |
| Chromosome: | chromosome 12 |
| Location: | 5465284 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530700 | (1 of 1) K03126 - transcription initiation factor TFIID subunit 12 (TAF12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGTTGCAATCCGGCAACGCGCTAAGCT |
| Internal bar code: | AACGCCGGATTAAGTGGTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 98.8113 |
| LEAP-Seq n confirming: | 2577 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTCTACACCGAGTGGG |
| Suggested primer 2: | GACTCGGATGTGAGCTGTGA |