| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.169805 |
| Chromosome: | chromosome 8 |
| Location: | 2217440 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369800 | DHS2 | Deoxyhypusine synthase; (1 of 2) 2.5.1.46 - Deoxyhypusine synthase / Spermidine dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGTGTAGCCCAGGAAGATGATGCAGC |
| Internal bar code: | TTAGCTAACGAAGGCTGTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.2828 |
| LEAP-Seq n confirming: | 1938 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAATGGTGAGTTTACGC |
| Suggested primer 2: | AGTAGTTGGCGTTGGGAATG |