| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.169840 |
| Chromosome: | chromosome 11 |
| Location: | 3189851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479416 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGAGATCGCCGTCCTGAGCGTCAAGG |
| Internal bar code: | GACAGTGGGGGGGTACACGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1159 |
| LEAP-Seq percent confirming: | 75.4327 |
| LEAP-Seq n confirming: | 1351 |
| LEAP-Seq n nonconfirming: | 440 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCATCCTAAGCCAAAG |
| Suggested primer 2: | AGTTCACCGCCTACAAGTGG |