| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.169844 |
| Chromosome: | chromosome 10 |
| Location: | 2171459 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCTCGATGCCGTTTACATGCTTGCGG |
| Internal bar code: | ACTTTATTTAGTTGGCTGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1180 |
| LEAP-Seq percent confirming: | 98.6729 |
| LEAP-Seq n confirming: | 8476 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGTTGTTTCAGTGGGT |
| Suggested primer 2: | ATCCAGTCATCCCAGGTGAG |