| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169849 |
| Chromosome: | chromosome 7 |
| Location: | 758053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317908 | (1 of 1) K04485 - DNA repair protein RadA/Sms (sms, radA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCAGCCCGCTCGCCGAGTTCCCGCGCG |
| Internal bar code: | GTGCAGGGTCAATCGTGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.8501 |
| LEAP-Seq n confirming: | 1332 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCATTCACAGGTTTGCC |
| Suggested primer 2: | GGTCACAGGACGTCATTCCT |