| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.169857 |
| Chromosome: | chromosome 16 |
| Location: | 2833368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663350 | CLPX1,CLPX | ClpX chaperone; (1 of 1) K03544 - ATP-dependent Clp protease ATP-binding subunit ClpX (clpX, CLPX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCATTTCATTTGGTGGGTCGGTGAGG |
| Internal bar code: | GTGCGGGTGGAGTGCAATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.6124 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGAACCCTGTGGTGTT |
| Suggested primer 2: | CACGTGGGCTACAACAAATG |